d-柠檬烯诱导白血病细胞凋亡的机制研究

被引:13
作者
王金香
张玉祥
机构
[1] 广东食品药品职业学院
关键词
d-柠檬烯; Caspase-8活化; 白血病; 凋亡;
D O I
10.16661/j.cnki.1672-3791.2010.05.107
中图分类号
R285.5 [中药实验药理];
学科分类号
100806 [中药药理学];
摘要
探讨传统中药中挥发油成分d-柠檬烯在HL-60细胞株中的抗肿瘤作用机制。体外细胞培养、凋亡检测、流式细胞术和蛋白免疫印迹等分子生物学技术进行机制研究。d-柠檬烯可以抑制HL-60细胞增值的同时可以诱导HL-60细胞的凋亡。d-柠檬烯可引起细胞内活性氧(ROS)的蓄积、线粒体膜电位(MMP)的下降和Caspase-8活化。抗氧化剂N-乙酰半胱氨酸(NAC)可以阻断d-柠檬烯诱导的细胞内活性氧(ROS)的蓄积,但不能阻断线粒体膜电位(MMP)的下降和Caspase-8活化。d-柠檬烯诱导的HL-60细胞凋亡机制是通过活性氧非依赖的Caspase-8活化来实现的。
引用
收藏
页码:6 / 9
页数:4
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