快速提取肠道微生物基因组DNA的方法

被引：25

作者：

金晶

彭颖

李晓波

机构：

[1] 上海交通大学药学院

来源：

现代生物医学进展 | 2007年 / 01期

基金：

国家自然科学基金重大研究计划;

关键词：

肠道微生物; 简便酚/氯仿抽提法; ERIC-PCR; 16S r DNA-RFLP;

D O I：

10.13241/j.cnki.pmb.2007.01.035

中图分类号：

Q93-33 [微生物学技术与微生物学实验];

学科分类号：

071005 ; 100705 ;

摘要：

目的:肠道微生物的研究日益成为热点,如何获取高质量、较完整的肠道菌群基因组DNA是肠道微生物研究中的关键。本文通过对酚/氯仿法提取总DNA过程进行考察和优化,建立一种简便酚/氯仿抽提法。方法:考察和优化酚/氯仿法提取总DNA的过程,并根据DNA产量、纯度以及ERIC-PCR及16S rNDA-RFLP所反映的微生物群落结构特性的指标,并与QIAamp?DNA Stool Mini Kit提取的进行比较,评价了所建立的快速提取方法。结果:用简便酚/氯仿法得到基本完整的基因组DNA,ERIC-PCR和16S rDNA-RFLP结果与QIAamp?DNA Stool Mini Kit法基本相同。结论:该方法快速并成本低,适合肠道微生物研究中总DNA提取,尤其适合处理大批量的样品。

引用

页码：100 / 103

页数：4

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