尼罗罗非鱼与萨罗罗非鱼及其正反杂交后代的微卫星分析

被引：22

作者：

颜标

李思发

蔡完其

机构：

[1] 上海水产大学农业部水产种质资源与养殖生态重点开放实验室

来源：

水产学报 | 2007年 / 03期

关键词：

尼罗罗非鱼; 萨罗罗非鱼; 杂交后代; 基因组DNA; 微卫星标记;

D O I：

暂无

中图分类号：

S917.4 [水产动物学];

学科分类号：

摘要：

Using microsatellite markers with 10 pairs of primers,genetic structure of Oreochromis niloticus,Sarotherodon melanotheron and their hybrids were analyzed.The results suggested that:① Six pairs of primers showed a rich polymorphic,which can be used as genetic markers for the genetic diversity study of these tilapias.② The analysis based on the six pairs of primers showed that the average heterozygosity(H)ranged as:O.niloticus ♀× S.melanotheron♂(0.725),S.melanotheron ♀ × O.niloticus ♂(0.692),S.melanotheron(0.537),O.niloticus(0.485).The highest effective numbers of alleles(Ne)existed in O.niloticus♀×S.melanotheron♂(3.79),the lowest existed in S.melanotheron(1.92).The average polymorphism information content(PIC)of 4 tilapias was 0.584,the rank from high to low as,O.niloticus ♀ × S.melanotheron ♂(0.668),S.melanotheron ♀× O.niloticus ♂(0.643),O.niloticus(0.375),S.melanotheron(0.333).③ The fact of enhanced heterozigosity of hybrids suggested that there were the genetic basement of cross breeding in tilapia.

引用

页码：411 / 415

页数：5

共 14 条

[1] Electrophoretic identification and phylogenetic relationships of indigenous tilapiine species of Zimbabwe. Shoniwa F F,Howard J H. Journal of Fish Biology . 1998
[2] 微卫星标记OarAE101和BM1329在五个绵羊品种中的初步研究
储明星
程金华
过纬
[J]. 遗传学报, 2001, (06) : 510 - 517
[3] 尼罗罗非鱼品系间形态差异分析
李思发
李晨虹
李家乐
[J]. 动物学报, 1998, (04) : 75 - 82
[4] 分子克隆实验指南[M]. 科学出版社 , (美)J.萨姆布鲁克(JosephSambrook),(美)D.W.拉塞尔(DavidW.Russell)著, 2002
[5] Construction of a genetic linkage map in man using restriction fragment length polymorphism. Botstein D,White R L,Skolnick M. The American Journal of Human Genetics . 1980
[6] Development of polymorphic microsatellite DNA loci for characterizing Oreochromis shiranussubspecies in Malawi. Ambali AJ D,Doyle R W,Cook DI. Journal of Applied Ichthyology . 2000
[7] Genetic diversity and differentiation of Nile tilapia(Oreochromis niloticus)revealed by DNA microsatellites. HeshamA H,John G. Aquaculture Research . 2005
[8] Microsatellite variation associated with prolactin expression and groth of salt-challengedtilapia. Streelman J T,Kocher T D. Physiological Genomics . 2002
[9] Microsatellites for linkage analysis of genetic traits. Heame C M,Ghosb S,Todd J A. Trends in Genetics . 1992
[10] Genetic changes inOreochromis shiranus(Trewavas)associated with the early stages of national aquaculture development in Malawi. Ambali AJ D,Doyle R W,Cook DI. Aquaculture Research . 1999

← 1 2 →