环介导等温扩增法检测牛羊肉中的猪肉成分

被引：8

作者：

杨丽霞

淑君

彭新凯

机构：

[1] 长沙市食品质量安全监督检测中心

来源：

食品与机械 | 2013年 / 29卷 / 05期

关键词：

环介导等温扩增; 猪肉; 快速检测;

D O I：

暂无

中图分类号：

TS251.7 [产品标准与检验];

学科分类号：

摘要：

研究建立实时荧光环介导等温扩增方法,以实现对牛羊肉中猪肉成分的快速检测。针对猪肉线粒体DNACOXI基因设计LAMP引物,通过扩增产物电泳和终点染色法鉴定反应,同时采用荧光检测仪实时监控反应过程。结果表明,LAMP法能有效、特异地检测出牛羊肉中的猪肉成分,该方法快速、简便、灵敏度高,可方便的应用到肉制品检测中。

引用

页码：63 / 65

页数：3

共 14 条

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