水稻叶绿体基因组中一个编码psbL和psbJ基因cDNA的克隆与分析

被引：1

作者：

顾克余

罗林广

苏昌潮

翟虎渠

机构：

[1] 南京农业大学作物遗传与特异种质创新教育部重点实验室!南京,南京农业大学作物遗传与特异种质创新教育部重点实验室!南京,南京农业大学作物遗传与特异种质创新教育部重点实验室!南京,南京农业大学作物遗传与特异种质创新教育部重点实验室!南京

来源：

植物学报 | 2001年 / 02期

关键词：

水稻; psbL和psbJ; cDNA克隆; 基因表达; 差异显示;

D O I：

暂无

中图分类号：

Q943.2 [植物基因工程];

学科分类号：

071007 ; 090102 ;

摘要：

A 505 bp cDNA was cloned from the leaves of rice (Oryza sativa L.) Shanyou 63 combination. DNA sequence analysis showed that it is a part of rice chloroplast genome. Its homology comparison with those known in GenBank found that it encodes 38 amino acid peptide deduced from psbL gene and 40 amino acid peptide deduced from psbJ gene in rice chloroplast PSⅡ. Northern hybridization showed that the cDNA was differentially displayed in hybrid F 1 and its parental lines.

引用

页码：210 / 212

页数：3

共 2 条

[1]

The complete sequence of the rice （ Oryza sativa ） chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals[J] . Junzou Hiratsuka,Hiroaki Shimada,Robert Whittier,Takashi Ishibashi,Masahiro Sakamoto,Masao Mori,Chihiro Kondo,Yasuko Honji,Chong-Rong Sun,Bing-Yuan Meng,Yu-Qing Li,Akira Kanno,Yoko Nishizawa,Atsushi Hirai,Kazuo Shinozaki,Masahiro Sugiura.MGG Molecular ＆ General Genetics . 1989 (2)

[2]

Sambrook,J,Fritsch,EF,Maniatis,T. Molecular Cloning: A laboratory Manual . 1989

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