PCR扩增特异性16SrDNA和溶血素基因检测致病性嗜水气单胞菌

被引：33

作者：

储卫华

陆承平

机构：

[1] 南京农业大学农业部动物疫病诊断与免疫重点实验室

[2] 南京农业大学农业部动物疫病诊断与免疫重点实验室江苏南京　

[3] 江苏南京　

来源：

水产学报 | 2005年 / 01期

关键词：

嗜水气单胞菌; 16SrDNA; 气溶素基因; 聚合酶链式反应; 检测;

D O I：

暂无

中图分类号：

S941 [鱼病学];

学科分类号：

摘要：

根据已发表的气单胞菌16SrDNA基因序列及气单胞菌气溶素(aerolysin)基因序列,设计了2对引物,建立了检测致病性嗜水气单胞菌的PCR方法。通过对12株气单胞菌的检测,发现16SrDNA引物具有高度的特异性,仅对嗜水气单胞菌扩增阳性。而Aero基因引物检测结果与采用生物学方法(鲜血平板法)检测的结果符合率为97.2%,且具有高度的敏感性,可检测最低1fg的模板。将16SrDNA与Aero基因结合PCR方法检测致病性嗜水气单胞菌与用致病性嗜水气单胞菌检测试剂盒的符合率为94.4%。该方法的建立为致病性嗜水气单胞菌的检测提供了一种简便、快速的途径,是一种比较实用的致病性嗜水气单胞菌的检测方法。

引用

页码：79 / 82

页数：4

共 11 条

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陆承平 .

水产学报, 1992, (03) :282-288

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