罗非鱼体内氟甲砜霉素的高效液相色谱测定方法研究

被引:8
作者
冯敬宾
贾晓平
李刘冬
机构
[1] 中国水产科学研究院南海水产研究所农业部渔业生态环境重点开放实验室
[2] 中国水产科学研究院南海水产研究所农业部渔业生态环境重点开放实验室 广东 广州
[3] 上海水产大学海洋学院
[4] 上海
[5] 广东 广州
[6] 广东 广州
基金
广东省科技计划;
关键词
高效液相色谱测定方法; 氟甲砜霉素; 罗非鱼; 血浆; 肌肉; 肝脏;
D O I
暂无
中图分类号
S948 [水产药物学、药理学];
学科分类号
摘要
建立了罗非鱼(Oreochromis niloticus×O.aureus)血浆、肌肉和肝脏内氟甲砜霉素的高效液相色谱测定方法(HPLC法)。采用内标法和峰高法定量,方法准确,重复性好,灵敏度较高。8、1和0.125μg·mL-13个浓度水平的加标回收率为89.16%-96.13%;对应的批内精密度和批间精密度分别为2.21%-6.55%和2.11%- 7.71%。方法检出限为0.03μg·mL-1。结果表明,此方法非常适合用于罗非鱼体内氟甲砜霉素的动力学和残留研究。
引用
收藏
页码:35 / 42
页数:8
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