连翘苷对创伤性骨折大鼠模型骨愈合作用的研究

被引：10

作者：

张震

张庆峰

李伟

杨超群

刘斌礼

李德民

机构：

[1] 济南市第三人民医院脊柱科

来源：

免疫学杂志 | 2019年 / 01期

关键词：

连翘苷; 骨关节炎; 炎症因子; 骨愈合;

D O I：

10.13431/j.cnki.immunol.j.20190011

中图分类号：

R-332 [医用实验动物学]; R285.5 [中药实验药理];

学科分类号：

1001 ; 1008 ;

摘要：

目的本研究旨在探索连翘苷(forsythin)对大鼠软组织损伤和骨愈合作用的影响。方法利用闭合式股骨干骨折术模拟大鼠创伤性骨折模型,模拟创伤性骨关节炎。SD大鼠随机分为4组:健康对照组、健康加药组、骨折模型组和模型加药组。番红O快绿染色检测大鼠骨组织变化并检测大鼠血清Ca、P含量和碱性磷酸酶(ALP)、骨钙素(BGP)活性;Western blot检测血清骨形态发生蛋白(BMP-2)、胶原和增殖标记蛋白表达水平;ELISA检测炎症因子iNOS, TNF-α和IL-6水平。结果连翘苷减轻骨折后骨组织病理损伤;与模型组相比,连翘苷增加骨折后血清Ca、P含量、ALP和BGP活性、血清BMP-2、胶原和增殖标记蛋白表达水平,减少骨折后血清炎症因子iNOS, TNF-α和IL-6水平。结论连翘苷可调节成骨指标和炎症因子,从而改善骨折软组织损伤,促进骨愈合。

引用

页码：71 / 76

页数：6

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