嗅鞘细胞联合血管内皮生长因子促进周围神经再生的实验研究

被引：2

作者：

王磊

吕刚

李永明

庄培元

李帅

贾志强

机构：

[1] 辽宁医学院

来源：

山东医药 | 2012年 / 52卷 / 33期

关键词：

神经再生; 嗅鞘细胞; 血管内皮生长因子; 移植;

D O I：

暂无

中图分类号：

R651.3 [周围神经];

学科分类号：

1002 ; 100210 ;

摘要：

目的探讨嗅鞘细胞(OECs)与血管内皮生长因子(VEGF)联合移植促进坐骨神经再生的协同作用。方法取SD大鼠60只,随机分成4组,即生理盐水(CON)组、VEGF组、OECs组和VEGF+OECs组。制作大鼠坐骨神经缺损模型,在再生室内分别注入CON、VEGF、OECs、VEGF+OECs。术后观察各组大鼠一般状态,并于术后4周和12周在光镜、电镜下观察再生室神经再生情况,检测大鼠坐骨神经电生理情况等。结果 VEGF组、OECs组和VEGF+OECs组坐骨神经单位横断面有髓神经纤维计数及其直径,电生理检测结果及腓肠肌湿质量均高于CON组(P均<0.05)。VEGF+OES组神经纤维的组织形态学优于VEGF、OECs组。结论 OECs移植联合VEGF对大鼠神经再生均有明显的促进作用,二者有协同作用。

引用

页码：25 / 27

页数：3

共 15 条

[1]

Neogenin:one receptor,many functions. Wilson NH,Key B. International Journal of Biochemistry Cell Biology The . 2007

[2]

Neogenin:A multi-functional receptor regulating diverse developmental processes. Cole SJ,Bradford D,Cooper HM. International Journal of Biochemistry Cell Biology The . 2007

[3]

Emerging roles for neogenin and its ligands in CNS development. De Vries M,Cooper HM. Journal of Neurochemistry . 2008

[4]

Myosin X regulates netrin receptors and functions in axonal path-finding. Zhu XJ,Wang CZ,Dai PG et al. Nature Cell Biology . 2007

[5]

Peripheral nerve grafts promoting central nervous system regeneration after spinal cord injury in the primate. Levi A D,Dancausse H,Li X,et al. Journal of Neurosurgery . 2002

[6]

Paracrine and autocrine functions of neuronal vascular endothelial growth factor (VEGF) in the central nervous system. Ogunshola O O,Antic A,Donoghue M J,et al. Journal of Biological Chemistry . 2002

[7]

Transplantation of nasal olfactory tissue promotes partial recovery in paraplegic adult rats. Lu J,Feron F,Ho S M,et al. Brain Research . 2001

[8]

Olfactory ensheathing cells do not require L-ascorbic acid in vitro to assemble a basal lamina or to myelinate dorsal root ganglion neurites. Devon R,Doucette R. Brain Research . 1995

[9]

Vascular endothelial growth factor is a secreted angiogenic mitogen. Leung DW,Cachianes G,Kuang WJ,et al. Science . 1989

[10]

Olfactory ensheathing cells do not require l -ascorbic acid in vitro to assemble a basal lamina or to myelinate dorsal root ganglion neurites[J] . R. Devon,R. Doucette. &nbspBrain Research . 1995 (1)

← 1 2 →