大白菜表达序列标签SSR标记分析

被引：34

作者：

葛佳

谢华

崔崇士

洪剑明

马荣才

机构：

[1] 北京农业生物技术研究中心

[2] 东北农业大学园艺学院

[3] 首都师范大学生命科学学院

来源：

农业生物技术学报 | 2005年 / 04期

关键词：

EST; EST-SSR; 大白菜;

D O I：

暂无

中图分类号：

S634.1 [大白菜];

学科分类号：

摘要：

对大白菜(BrassicacampestrisL.ssppekinensis)13007个EST序列进行拼接共得到7714个片段重叠群(contig),其中10.4%(890个)非冗余EST含有SSR标记(EST-SSR),标记间平均间隔距离为3.9kb,单、双和三核苷酸重复序列大约各占1/3左右。CDS所包含的三核苷酸重复序列最多,而且AAG/CTT重复序列的含量最高。通过同源性比较分析,获得功能已知的SSR-ESTs774个,共计含有846个SSR,其中50.2%位于5'-UTR,31.4%位于3'-UTR,18.4%位于CDS。实验选取123个SSR座位设计引物,利用芜菁花叶病毒(Turnipmosaicvirus,TuMV)的高抗和高感大白菜F6代自交系材料各2份,分析EST-SSR标记多态性。结果表明,其中37个标记可进行有效扩增,但是没有表现出多态性,23个标记(18.7%)至少在1份材料中具有多态性。对这些EST-SSR标记在大白菜和其它芸薹属植物的系统发育关系分析、遗传作图和基因定位的应用进行了讨论。

引用

页码：423 / 428

页数：6

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