二甲双胍对高糖培养H9c2细胞Connexin43表达的影响

被引：4

作者：

王光宇

毕亚光

刘向东

魏盟

张庆勇

机构：

[1] 上海交通大学附属第六人民医院心血管内科

来源：

中国药理学通报 | 2016年 / 32卷 / 07期

关键词：

二甲双胍; 高血糖; connexin43; 氧化应激; AMP依赖的蛋白激酶; H9c2;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的研究二甲双胍(metformin,Met)对高糖培养下的H9c2细胞缝隙连接蛋白43(connexin43,Cx43)表达的影响及其机制。方法高糖培养的大鼠H9c2心肌细胞分别加入3和5μmol·L-1的两种不同浓度的二甲双胍继续培养24 h。MTT实验检测H9c2细胞活力;LDH释放实验检测细胞毒力;细胞免疫荧光实验检测Cx43的表达和分布;荧光法检测细胞内活性氧(ROS)水平;Western blot检测Cx43、pAMPK、AMPK和GAPDH的表达。结果 Met能增加H9c2细胞活力,降低细胞内ROS水平,对LDH释放差异无显著性;Met使AMPK磷酸化水平明显升高,增加心肌Cx43表达,改善Cx43的分布。结论 Met可能通过激活AMPK途径,增加心肌细胞Cx43的表达和减少细胞内ROS的产生,进而发挥心血管的保护效应。

引用

收藏

页码：920 / 924

页数：5

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