多种基因分型技术在识别结核分枝杆菌基因型的联合应用

被引：2

作者：

胡屹

蒋伟利

赵琦

王伟炳

徐飚

机构：

[1] 复旦大学公共卫生学院流行病学教研组

来源：

中华结核和呼吸杂志 | 2009年 / 08期

关键词：

分枝杆菌,结核; 串联重复序列; 限制性内切酶图谱法; 基因型;

D O I：

暂无

中图分类号：

R686 [筋腱、韧带、滑囊疾病及损伤];

学科分类号：

1002 ; 100210 ;

摘要：

目的评价MTB散在分布重复单位(MIRUs)和IS6110限制性内切酶片段长度的多态性(IS6110-RFLP)基因分型技术,以及两者联合应用在我国华东农村地区MTB分子流行病学研究中的应用及其效果。方法以人群为基础的横断面研究设计,采用比例法确定2004—2005年从浙江省德清县和江苏省灌云县收集的351株MTB菌株的耐药性,利用间隔区寡核苷酸分型方法识别北京基因型MTB,并进行MIRUs和IS6110-RFLP独立基因分型和串联联合应用。结果先对351株MTB菌株采用MIRUs基因分型可以识别235种基因型,包括46个成簇基因型和189个惟一基因型,分辨力为0.9317;用IS6110-RFLP可进一步从成簇基因型中识别出28个亚组,分辨力为0.9989。相对而言,先对目标菌株采用IS6110-RFLP基因分型,可识别267基因型,分辨力为0.9684,包括46个成簇基因型和221个惟—基因型,再用MIRUs基因分型技术可进一步从成簇基因型中识别出31个亚型,分辨力为0.9992。两种顺序不同联合分型方法的基因分型的辨别能力相似,尤其是在耐多药MTB(分辨力分别为0.9965和0.9963)及北京基因型MTB(分辨力分别为0.9930和0.9933)的分型中。结论MIRUs分型的方法简便、快速,结果有效、可靠,在大规模的结核病分子流行病学研究中,可先采用MIRUs,再对成簇MTB菌株进行IS6110-RFLP二次分型的联合分型策略。

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