番茄细菌性溃疡病菌的实时荧光PCR检测

被引：22

作者：

夏明星 ^{[1
]}

赵文军 ^{[2
]}

马青 ^{[3
]}

朱水芳 ^{[2
]}

机构：

[1] 黄岛出入境检验检疫局

[2] 中国检验检疫科学研究院动植物检疫研究所

[3] 西北农林科技大学

来源：

植物病理学报 | 2006年 / 02期

关键词：

实时荧光PCR; 番茄细菌性溃疡病菌; ITS; TaqMan探针;

D O I：

10.13926/j.cnki.apps.2006.02.009

中图分类号：

S436.412 [番茄病虫害];

学科分类号：

摘要：

由C lavibacter m ichiganensissubsp.m ichiganensis(Cmm)引起的番茄细菌性溃疡病是一种严重危害番茄生产的种传细菌性病害。根据ITS序列多态性设计引物及TaqM an探针进行实时荧光PCR检测的结果表明,这组引物-探针能检测出所有供试的Cmm菌,对照菌均未检测到荧光信号。用接种但未显示症状的番茄苗叶片及人工处理的带菌种子提取的核酸作为模板,均能检测到病菌,其检测灵敏度比常规PCR高约100倍。实验中不需病原菌的分离培养及PCR的后续处理。该方法快速、简便、安全、准确,适用于出入境检验检疫及种子、种苗健康检测领域。

引用

页码：152 / 157

页数：6

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