革兰阴性杆菌中质粒ampC基因的检测与分析

被引:32
作者
蒋燕群
曹娜
陈泰尧
汤瑾
倪语星
机构
[1] 上海交通大学附属第六人民医院检验科
[2] 上海第二医科大学附属瑞金医院检验科
关键词
质粒; ampC基因; 多重聚合酶链反应;
D O I
暂无
中图分类号
R446.5 [微生物学检验];
学科分类号
摘要
目的 运用多重聚合酶链反应 (PCR)检测革兰阴性杆菌的质粒ampC基因。 方法 通过热裂解获得DNA模板 ,用 6组引物进行多重PCR扩增 ,用琼脂糖凝胶电泳区分PCR产物。结果  2 0 0株临床分离的革兰阴性杆菌中有 6株为质粒ampC基因阳性。 结论 用多重PCR技术能简单、快速地检测革兰阴性杆菌质粒ampC基因 ,且特异性高。
引用
收藏
页码:328 / 330
页数:3
相关论文
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