大白菜无菌苗叶肉原生质体植株再生

被引：6

作者：

李世君

孟征

盛方镜

李德葆

机构：

[1] 浙江农业大学生物技术研究所

来源：

生物工程学报 | 1992年 / 03期

关键词：

大白菜; 原生质体; 植株再生;

D O I：

10.13345/j.cjb.1992.03.008

中图分类号：

学科分类号：

摘要：

商用大白菜叶肉原生质体,经液体培养基浅层培养,再生细胞分裂,并获得愈伤组织。愈伤组织转到分化培养基上诱导分化,已从城青2号大白菜叶肉原生质体得到再生的完整植株。再生细胞的分裂频率与培养基中的激素种类和浓度有关,受原生质体培养浓度的影响。多胺类物质[亚精胺(SPD)]的加入,可促进细胞分裂,并有益于以后植株的再生。通过提高新鲜培养液的pH值(6.5)可使细胞团的褐化得到明显的控制。在植株分化过程中,培养基中低浓度(1.1%)的蔗糖与植株分化有关。

引用

页码：249 / 254+311 +311

页数：7

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