成人骨髓间质干细胞定向诱导为神经元样细胞的研究

被引：52

作者：

项鹏

夏文杰

张丽蓉

陈振光

张秀明

李艳

李树浓

机构：

[1] 中山医科大学病理生理教研室!广东广州,中山医科大学病理生理教研室!广东广州,广州药学院病理生理教研室!广东广州,中山医科大学附属第一医院心胸外科!广东广州,中山医科大学病理生理教研室!广东广州,中山医科大学病理生理教研室!广东广州

来源：

中国病理生理杂志 | 2001年 / 05期

关键词：

干细胞; 神经元; 神经系统疾病;

D O I：

暂无

中图分类号：

R329.2 [人体细胞学];

学科分类号：

摘要：

目的 :体外定向诱导成人骨髓间质干细胞 (MSC)分化为神经元样细胞。方法 :采用Ficoll -Paque液 (1 0 77× 10 3 g/L)离心分离成人MSC ,体外扩增 ,分别采用含巯基乙醇和硫代甘油等试剂的无血清DMEM诱导MSC分化为神经元。免疫组化鉴定神经元烯醇化酶 (NSE)、神经丝蛋白 (NF)、胶质纤维酸性蛋白 (GFAP)的表达。结果 :成人骨髓间质干细胞在体外扩增 5代可获 5× 10 7个细胞。加入巯基乙醇等或硫代甘油等诱导剂诱导后 ,MSC胞体收缩 ,突起伸出 ;免疫组化显示诱导出的神经元样细胞NSE、NF表达阳性 ,GFAP阴性。结论 :成人骨髓间质干细胞在体外可以分化为神经元样细胞。

引用

页码：2 / 4+98

页数：4

共 9 条

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