脑缺血再灌注后神经细胞凋亡的研究

被引:1
作者
吴卫平
李振洲
匡培根
机构
[1] 武警总医院神经内科!北京
[2] 解放军总医院神经内科
关键词
脑缺血; 再灌注; 凋亡;
D O I
10.14010/j.cnki.wjyx.2001.03.004
中图分类号
R363 [病理生理学];
学科分类号
100104 ;
摘要
目的 通过大鼠脑缺血再灌注模型 ,观察缺血 1 5h后 ,再灌注 1 2、2 4、4 8和 72h皮层、尾壳核和海马细胞凋亡的变化。方法 采用线栓法制成大鼠左侧大脑中动脉缺血再灌注模型 (n =2 1 ) ,应用末端转移酶介导的dUTP -DIG缺口末端标记法 (TUNEL) ,标记凋亡细胞断裂的DNA。结果 脑缺血再灌注后 1 2h,左侧大脑中动脉缺血区皮层和尾壳核出现大量凋亡细胞 ,于再灌注后 2 4~ 4 8h达高峰。结论 缺血再灌注早期可诱发细胞凋亡
引用
收藏
页码:140 / 143
页数:4
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