嗜麦芽窄食单胞菌处理苯并[a]芘-铜复合污染过程中细胞表面特性的变化

被引:6
作者
陈烁娜 [1 ,2 ]
尹华 [1 ,2 ]
叶锦韶 [1 ,2 ]
彭辉 [2 ]
张娜 [1 ,2 ]
何宝燕 [1 ,2 ]
机构
[1] 暨南大学环境工程系
[2] 广东省高校毒害性污染物防治与生物修复重点实验室
关键词
嗜麦芽窄食单胞菌; 复合污染; 细胞疏水性; 质膜通透性;
D O I
暂无
中图分类号
X171.5 [污染生态学];
学科分类号
071012 ; 0713 ;
摘要
利用嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia)对BaP-Cu进行降解/吸附实验,考察菌体处理污染物过程中细胞表面特性的变化。结果表明,S.maltophilia细胞表面具有较高的疏水性。从4h到72h,不同污染体系中S.maltophilia的CSH均维持在83.3%~95.6%,之后随着时间的推移,CSH开始逐渐降低。污染物可以使S.maltophilia细胞质膜的通透性增加。TEM检测结果证实处理污染物之后,菌体质膜通透性改变,破裂并出现了"孔洞",导致细胞质外流,但是细胞壁依然维持细胞形状而没有完全裂解。处理了复合污染物的菌体,到第5d细胞严重变形,周质空间变大,细胞质外泄严重,而且原核分解并逐渐消失,细胞活性降低,并逐渐丧失对重金属的吸附能力。
引用
收藏
页码:1592 / 1598
页数:7
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