大气细颗粒物水溶成分和非水溶成分的细胞毒性

被引：19

作者：

曹强 ^{[1
]}

钱孝琳 ^{[1
]}

张澍 ^{[2
]}

宋伟民 ^{[1
]}

机构：

[1] 复旦大学公共卫生学院环境卫生教研室

[2] 上海国际机场检验检疫局

来源：

环境科学学报 | 2008年 / 06期

基金：

国家自然科学基金重大项目;

关键词：

细颗粒物; 水溶成分; 非水溶成分; 活性氧;

D O I：

10.13671/j.hjkxxb.2008.06.021

中图分类号：

X513 [粒状污染物];

学科分类号：

摘要：

为了研究细颗粒物不同成分细胞毒作用的差异,以细颗粒物水溶成分和非水溶成分对人肺癌上皮细胞株A549细胞染毒.结果表明,在染毒剂量为100μg·mL-1、200μg·mL-1和300μg·mL-1的范围内,水溶成分的细胞毒性明显高于非水溶成分(P<0.05),表现为抑制细胞活性,导致细胞膜损伤增加,并且诱导细胞活性氧(ROS)的产生.使用N-乙酰半胱氨酸(NAC)和抗坏血酸(VitC)阻断活性氧(ROS)产生的研究结果表明,与未加上述抗氧化剂的染毒组相比,加抗氧化剂的染毒组细胞的活性明显增强,因此,提示细颗粒物水溶成分是最主要的毒性成分之一,而氧化-应激损伤可能是细颗粒物细胞毒作用的重要机制之一.

引用

页码：1167 / 1172

页数：6

共 9 条

[1] N-乙酰半胱氨酸的研究进展
李毅敏
赵树仪
[J]. 天津药学, 2003, (02) : 50 - 53
[2] Comparison of the sensitivity of three lung derived cell lines to metals from combustion derived particulate matter
Riley, MR
Boesewetter, DE
Turner, RA
Kim, AM
Collier, JM
Hamilton, A
[J]. TOXICOLOGY IN VITRO, 2005, 19 (03) : 411 - 419
[3] Effects of metals Cu, Fe, Ni, V, and Zn on rat lung epithelial cells[J] . Mark R. Riley,Dianne E. Boesewetter,Aana M. Kim,Francisco P. Sirvent.Toxicology . 2003 (3)
[4] Confounding and Effect Modification in the Short-Term Effects of Ambient Particles on Total Mortality: Results from 29 European Cities within the APHEA2 Project[J] . Klea Katsouyanni,Giota Touloumi,Evangelia Samoli,Alexandros Gryparis,Alain Le Tertre,Yannis Monopolis,Giuseppe Rossi,Denis Zmirou,Ferran Ballester,Azedine Boumghar,Hugh Ross Anderson,Bogdan Wojtyniak,Anna Paldy,Rony Braunstein,Juha Pekkanen,Christian Schindler,Joel Schwartz.Epidemiology . 2001 (5)
[5] Insoluble components of concentrated air particles mediate alveolar macrophage responses in vitro
Imrich, A
Ning, YY
Kobzik, L
[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2000, 167 (02) : 140 - 150
[6] Heart rate variability associated with particulate air pollution
Pope, CA
Verrier, RL
Lovett, EG
Larson, AC
Raizenne, ME
Kanner, RE
Schwartz, J
Villegas, M
Gold, DR
Dockery, DW
[J]. AMERICAN HEART JOURNAL, 1999, 138 (05) : 890 - 899
[7] Particulate and ozone pollutant effects on the respiratory function of children in southwest Mexico City
Gold, DR
Damokosh, AI
Pope, CA
Dockery, DW
McDonnell, WF
Serrano, P
Retama, A
Castillejos, M
[J]. EPIDEMIOLOGY, 1999, 10 (01) : 8 - 16
[8] Oxidant generation and lung injury after particulate air pollutant exposure increase with the concentrations of associated metals
Pritchard, RJ
Ghio, AJ
Lehmann, JR
Winsett, DW
Tepper, JS
Park, P
Gilmour, MI
Dreher, KL
Costa, DL
[J]. INHALATION TOXICOLOGY, 1996, 8 (05) : 457 - 477
[9] Environmental Health Perspectives[C]. 2010 the 5th International Academic Conference on Environmental and Occupational Medicine,2010

← 1 →