黑曲霉改良株C112产β-葡萄糖苷酶的诱导及条件优化

被引：13

作者：

先天敏

陈介南

张林

机构：

[1] 中南林业科技大学国家林业局生物乙醇研究中心,生物环境科学与技术研究所

来源：

中南林业科技大学学报 | 2013年 / 33卷 / 11期

关键词：

黑曲霉; 发酵β-葡萄糖苷酶; 条件优化; 麦芽浸粉;

D O I：

10.14067/j.cnki.1673-923x.2013.11.004

中图分类号：

TQ925 [酶制剂（酵素）];

学科分类号：

083606 [微生物发酵工程];

摘要：

利用单因素实验及均匀设计法研究了黑曲霉C112发酵产β-葡萄糖苷酶的发酵条件。结果表明:黑曲霉C112发酵产β-葡萄糖苷酶的影响因素很多,包括接种方式(菌株活力)、接种量、碳氮源种类与浓度、发酵温度时间、初始pH值等。研究发现,通过种子接种改善菌株活力能有效提高产酶活力。碳源粒径也是影响诱导产酶效果的重要条件之一,当玉米芯粒径高于0.180 mm时产酶效果最佳。该菌株最适宜产酶条件为:玉米芯与麦芽浸粉(7:3)6 g/L、初始pH值4.87、30℃下发酵120 h,所得酶活力最大可达12.925 U/mL,较优化前提高了79.0%。

引用

页码：154 / 161

页数：8

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