应用代谢组学方法构建原发性肾病综合征诊断模型

被引：10

作者：

张晓波 ^{[1
]}

李鞠 ^{[2
]}

乔善磊 ^{[3
]}

夏彦恺 ^{[3
]}

唐凤英 ^{[1
]}

机构：

[1] 江苏省淮安市, 南京医科大学附属淮安第一医院肾内科

[2] 江苏省淮安市,

来源：

中华肾脏病杂志 | 2016年 / 05期

关键词：

肾病综合征; 诊断技术和方法; 代谢组学;

D O I：

暂无

中图分类号：

R692 [肾疾病];

学科分类号：

摘要：

目的应用代谢组学的方法分析原发性肾病综合征(primary nephrotic syndrome, PNS)患者血清的代谢物特征并构建疾病血清代谢物诊断模型, 寻找与疾病相关的代谢通路。方法选取2010年12月至2012年4月在南京医科大学附属淮安第一医院就诊的30例PNS患者。应用液相色谱质谱联用技术(LC-MS)方法对PNS患者和健康志愿者(n=30)血清样本进行代谢物无靶标检测, 采集代谢物指纹图谱, 结合模式识别分析方法构建PNS诊断模型, 并进行MetPA代谢通路分析。结果主成分分析法(PCA)、非监督的偏最小二乘法-判别分析(PLS-DA)均显著区分PNS组与健康对照组, PLS-DA模型的预测率Q2=0.300, 解释性良好(R2X=0.581, R2Y=0.452)。与健康对照组比较, PNS患者血清胆甾烷3, 7, 12, 15醇、二酰甘油、植物鞘氨醇和色氨酸均降低, 鞘磷脂、精氨酸和谷氨酸均增加(均VIP>1且P<0.05);PNS患者血清代谢紊乱通路主要包括鞘脂类代谢、精氨酸脯氨酸代谢、亚油酸代谢、嘧啶代谢(影响因子>0.10, 均P<0.05)。结论代谢组学技术结合模式识别分析方法有望成为临床诊断PNS及病情监测的新方法。

引用

页码：334 / 338

页数：5

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