疏肝利胆方干预胆色素结石模型豚鼠的作用机制研究

被引：6

作者：

刘莹露

王玉娟

徐天舒

机构：

[1] 南京大学医学院附属鼓楼医院

来源：

河南中医 | 2017年 / 37卷 / 03期

关键词：

疏肝利胆方; 胆色素结石; 细胞因子; 调节免疫; 豚鼠;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理]; R-332 [医用实验动物学];

学科分类号：

100402 [劳动卫生与环境卫生学]; 100806 [中药药理学];

摘要：

目的:探讨疏肝利胆方干预胆色素结石模型豚鼠的作用机制。方法:将15只豚鼠随机分为正常组、模型组及中药组,每组各5只。空白组和模型组每只豚鼠给予生理盐水1 m L·d-1灌胃,中药组给予疏肝利胆方灌胃。观察三组豚鼠成石率,并用酶联免疫吸附试验(EnzymeLinked Immuno Sorbent Assay,ELISA)测定豚鼠血清中白介素-15(Interleukin-15,IL-15)、IL-1β、IL-2、前列腺素E2(Prostaglandin E2,PGE2)及胆汁中IL-15、IL-2、C反应蛋白(C reactive protein,CRP)的含量。结果:中药组胆色素结石成石率较模型组明显下降,差异有统计学意义(P<0.05);与模型组比较,中药组中血清IL-15、IL-1β、PGE2的含量减少,IL-2的含量增加,差异均有统计学意义(P<0.05);与模型组比较,中药组胆汁IL-15、CRP的含量减少,IL-2的含量增加,差异具有统计学意义(P<0.05)。结论:疏肝利胆方可减少胆色素结石成石率,其治疗胆色素结石机制与下调某些促炎细胞因子、调节免疫机理有关。

引用

页码：417 / 420

页数：4

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