血管紧张素Ⅱ对人内皮细胞转录因子NF-κB的激活机制及其对血小板源生长因子B链基因转录的影响

被引:8
作者
李皓
尹鸿操
张华
王宗立
佘铭鹏
机构
[1] 中国协和医科大学基础医学研究所病理研究室,中国协和医科大学基础医学研究所病理研究室,中国协和医科大学基础医学研究所病理研究室,中国协和医科大学基础医学研究所病理研究室,中国协和医科大学基础医学研究所病理研究室
关键词
血管紧张素Ⅱ; 内皮,血管; NF-κB; 血小板源生长因子;
D O I
暂无
中图分类号
R363 [病理生理学];
学科分类号
100104 ;
摘要
目的 研究血管紧张素Ⅱ对ECV3 0 4细胞中转录因子NF κB的作用和对血小板源生长因子B链 (PDGF B)基因表达的影响。方法 采用电泳迁移率移动分析法 (EMSA) ,免疫组织化学方法 ,共聚焦显微镜及金颗粒标记免疫电镜技术 ;荧光素酶报告基因与变异型激酶质粒共转染的方法及Northern印迹法研究血管紧张素Ⅱ激活ECV3 0 4细胞NF κB的信号传递路径和检测了血管紧张素Ⅱ刺激前后ECV3 0 4细胞PDGF BmRNA的表达水平。结果 血管紧张素Ⅱ刺激后 ,在ECV3 0 4细胞内有NF κB的激活及核易位过程 ,应用免疫荧光共聚焦显微镜 ,免疫电镜及Northern印迹等方法均可观察到PDGF B或其基因表达增高。变异型激酶质粒IKKα KM ,IKKβ KM及NIK Km可抑制经血管紧张素Ⅱ刺激的转染细胞内与NF κB启动相连的荧光素酶的表达。结论 血管紧张素Ⅱ可激活胞质内NF κB并出现核易位 ,激酶NIK、IKKα 和IKKβ 参与了此信号传递路径。血管紧张素Ⅱ刺激后PDGF B链mRNA水平增高。
引用
收藏
页码:35 / 39
页数:5
相关论文
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