MALDI-TOF MS在临床微生物样本直接检测中的应用

被引：14

作者：

陈飞

胡玢婕

赵虎

机构：

[1] 复旦大学附属华东医院检验科

来源：

检验医学 | 2015年 / 30卷 / 07期

关键词：

基质辅助激光解吸电离飞行时间质谱; 直接检测; 微生物鉴定; 临床样本;

D O I：

暂无

中图分类号：

R446.5 [微生物学检验];

学科分类号：

摘要：

基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)是近年来发展起来的一项新兴的微生物鉴定技术,与传统的生化表型鉴定方法和分子生物学方法相比,MALDI-TOF MS具有操作简单、快速、准确和经济的特点。鉴于其对培养出的纯菌落鉴定的准确性和稳定性,MALDI-TOF MS也可被应用于临床样本的直接检测,包括阳性血培养瓶、中段尿、脑脊液等。本文主要就MALDI-TOF MS用于直接检测临床样本的原理、流程、鉴定效能及其研究进展等方面做一综述。

引用

页码：750 / 756

页数：7

共 19 条

[1] 分离胶促凝管联合MALDI-TOF MS直接检测血培养阳性细菌 [J].

陈峰 ;

李媛睿 ;

皇甫昱婵 ;

陶晓勤 ;

刘瑛 .

检验医学, 2015, 30 (02) :113-121

[2] MALDI-TOF MS在中段尿样本细菌直接检测中的应用 [J].

王琳 ;

李佳萍 ;

陈功祥 ;

张嵘 .

检验医学, 2015, 30 (02) :108-112

[3] Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium [J].

Idelevich, E. A. ;

Schuele, I. ;

Gruenastel, B. ;

Wuellenweber, J. ;

Peters, G. ;

Becker, K. .

CLINICAL MICROBIOLOGY AND INFECTION, 2014, 20 (10) :1001-1006

[4]

Comparative analysis of Gram's stain, PNA ‐ FISH and Sepsityper with MALDI ‐ TOF MS for the identification of yeast direct from positive blood cultures[J] . Rebecca L. Gorton,P. Ramnarain,K. Barker,N. Stone,S. Rattenbury,T. D. McHugh,C. C. Kibbler.Mycoses . 2014 (10)

[5]

Comparison between MALDI-TOF MS and FilmArray Blood Culture Identification panel for rapid identification of yeast from positive blood culture[J] . M. Paolucci,C. Foschi,M.V. Tamburini,S. Ambretti,T. Lazzarotto,M.P. Landini.Journal of Microbiological Methods . 2014

[6] Procedure for microbial identification based on Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry from screening-positive urine samples [J].

March Rossello, Gabriel A. ;

Gutierrez Rodriguez, Maria P. ;

de Lejarazu Leonardo, Raul Ortiz ;

Orduna Domingo, Antonio ;

Bratos Perez, Miguel A. .

APMIS, 2014, 122 (09) :790-795

[7]

Direct application of MALDI-TOF mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis[J] . Shunsuke Segawa,Setsu Sawai,Shota Murata,Motoi Nishimura,Minako Beppu,Kazuyuki Sogawa,Masaharu Watanabe,Mamoru Satoh,Tomoo Matsutani,Masayoshi Kobayashi,Yasuo Iwadate,Satoshi Kuwabara,Naokatsu Saeki,Fumio Nomura.Clinica Chimica Acta . 2014

[8] Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures [J].

Hong, Sung Kuk ;

Chang, Beung Ki ;

Song, Sang Hoon ;

Kim, Eui-Chong .

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2014, 32 (04) :419-422

[9]

New procedure for rapid identification of microorganisms causing urinary tract infection from urine samples by mass spectrometry (MALDI-TOF)[J] . Gabriel Alberto March Rosselló,María Purificación Gutiérrez Rodríguez,Raúl Ortiz de Lejarazu Leonardo,Antonio Ordu?a Domingo,Miguel ángel Bratos Pérez.Enfermedades Infecciosas y Microbiologia Clinica . 2014

[10]

Early identification of microorganisms in blood culture prior to the detection of a positive signal in the BACTEC FX system using matrix-assisted laser desorption/ionization–time of flight mass spectrometry[J] . Ming-Cheng Wang,Wei-Hung Lin,Jing-Jou Yan,Hsin-Yi Fang,Te-Hui Kuo,Chin-Chung Tseng,Jiunn-Jong Wu.Journal of Microbiology, Immunology and Infection . 2013

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