Use of fluorescent europium chelates as labels for detection of microcystin-LR in Taihu Lake, China

被引：5

作者：

钮伟民 ^{[1
]}

何恩奇 ^{[1
]}

吴庆刚 ^{[1
]}

周伟杰 ^{[1
]}

张艺 ^{[2
]}

黄飚 ^{[2
]}

赵晓联 ^{[3
]}

机构：

[1] Laboratory Department of Municipal Center for Disease Control and Prevention

[2] Key Laboratory of Nuclear Medicine, Department of Biotechnology, Jiangsu Institute of Nuclear Medicine

[3] Jiangsu Suwei Microbiology Research Co, Ltd

来源：

Journal of Rare Earths | 2012年 / 30卷 / 09期

关键词：

microcystin; time-resolved fluoroimmunoassay (TRFIA); water; dynamic detection; dead algae; rare earths;

D O I：

暂无

中图分类号：

X832 [水质监测];

学科分类号：

0804 ; 082803 ;

摘要：

A method using the time-resolved fluorescence technology to establish a highly sensitive microcystin-LR (MC-LR) indirect competitive immunoassay was proposed in this work. This method was used to monitor the MC-LR level in source water and treated drinking water from Taihu Lake. Algae in the water samples were removed by centrifugation, and the MC-LR level was quantified using this method. Testing results showed that the sensitivity of this method was 0.01 μg/L, and the dynamic measuring range was from 0.05 to 2 μg/L. The average recovery was 115%, and the variation (CV) within and between different batches were 7.3% and 9.7%, respectively. Testing results also indicated that this time-resolved fluoroimmunoassay was sensitive and accurate in measuring MC-LR level, especially for quantitative analysis MC-LR level in bulk water.

引用

页码：941 / 946

页数：6

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