促红细胞生成素预处理对大鼠脑缺血再灌注损伤的影响

被引：4

作者：

于代华

蒋玮

彭细娟

黎璞

朱江勃

机构：

[1] 第四军医大学唐都医院麻醉科

来源：

中华危重症医学杂志(电子版) | 2013年 / 6卷 / 06期

关键词：

促红细胞生成素; 缺血再灌注; 脑损伤;

D O I：

暂无

中图分类号：

R651.15 [];

学科分类号：

1002 ; 100210 ;

摘要：

目的研究促红细胞生成素(EPO)预处理对大鼠脑缺血再灌注损伤的影响。方法将30只Sprague-Dawley(SD)大鼠随机分为3组:假手术组、模型组、干预组,每组10只。干预组给予EPO预处理,15 min后,对模型组和干预组建立大脑中动脉栓塞模型,建模后2 h进行血流再灌注;对假手术组仅建立假手术模型,建模后2 h灌注生理盐水。记录各组大鼠神经功能缺陷评分、脑梗死面积、脑神经元凋亡情况,用RT-PCR检测谷氨酸转运体1(GLT-1)m RNA的表达量,免疫组化法检测谷氨酸-天门冬氨酸转运体(GLAST)蛋白表达。结果干预组大鼠神经功能缺陷评分和脑梗死灶面积明显小于模型组(P均<0.05)。与假手术组比较,模型组大量神经细胞凋亡,而干预组缺血区仅部分凋亡。脑缺血再灌注后2 h,模型组与干预组大鼠缺血区GLT-1 mRNA和GLAST蛋白表达均低于假手术组(P均<0.05),而干预组表达水平明显高于模型组(P均<0.05)。结论 EPO预处理可减轻大鼠脑缺血再灌注损伤。

引用

页码：339 / 343

页数：5

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