骨关节炎和类风湿性关节炎滑膜中IL-1受体拮抗剂三种同工型的表达及其生物学意义

被引:3
作者
裴明
于长隆
曲绵域
机构
[1] 北京大学第三医院运动医学研究所!
关键词
白介素1受体拮抗剂; 同工型; 表达; 骨关节炎; 类风湿性关节炎; 滑膜;
D O I
10.16038/j.1000-6710.2000.04.011
中图分类号
R593.22 [类风湿性关节炎];
学科分类号
1002 ; 100201 ;
摘要
白介素 1受体拮抗剂 (IL - 1Ra)以三种同工型的形式存在 ,即 17kDa的分泌型IL - 1Ra(sIL - 1Ra)、18kDa的细胞内IL - 1Ra(icIL - 1RaI)及 16kDa的细胞内IL - 1Ra(icIL - 1RaII)。本实验采用免疫组化和反转录PCR的方法 ,观察了IL - 1Ra的三种同工型在 3例骨关节炎 (OA)和 3例类风湿性关节炎 (RA)患者关节滑膜中的表达和分布情况。免疫组化结果显示OA和RA的滑膜中IL - 1Ra高度表达 ,主要集中在滑膜的衬里层细胞、炎症浸润细胞以及血管内皮细胞中 ;RT -PCR结果提示OA滑膜的三个标本中均只存在 16kDa的icIL - 1RaII型 ,而RA的三个滑膜标本中有两个存在IL - 1Ra的三种同工型 ,还有一个标本只存在 18kDa的icIL - 1RaI型。因此 ,OA和RA的滑膜中均高度表达IL -1Ra,但存在的形式和发挥作用的方式可能有所不同 ,推测与这两种疾病的发病机制有关。
引用
收藏
页码:368 / 370
页数:3
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