传染性支气管炎病毒核衣壳蛋白基因克隆及在E.coli中的表达

被引:7
作者
周继勇
丁红梅
程丽琴
沈行燕
机构
[1] 浙江大学动物预防医学研究所,浙江大学动物预防医学研究所,浙江大学动物预防医学研究所,浙江大学动物预防医学研究所杭州,杭州,杭州,杭州
基金
浙江省自然科学基金;
关键词
传染性支气管炎病毒; 核衣壳蛋白基因; 克隆; 非融合表达;
D O I
10.13865/j.cnki.cjbmb.2002.04.011
中图分类号
Q78 [基因工程(遗传工程)];
学科分类号
071007 ; 0836 ; 090102 ;
摘要
核衣壳蛋白基因 (N基因 )是传染性支气管炎病毒的重要结构基因 .根据已报道的序列设计引物 ,利用RT PCR技术从病毒RNA中扩增和克隆到了N基因的cDNA ,并测定了核苷酸序列 .克隆的N基因片段ORF全长 12 30bp ,编码 4 0 9个氨基酸 .将该片段序列与其他IBV病毒株比较 ,核苷酸的同一性为 87 0 %~ 98 6 %,氨基酸的同一性为 91 0 %~ 98 1%.将该cDNA亚克隆到pBV2 2 0表达载体 ,转化大肠杆菌DH5α菌株 ,Western印迹检测 ,获得了分子量约 4 5kD表达蛋白
引用
收藏
页码:450 / 455
页数:6
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