粒细胞-巨噬细胞集落刺激因子和干扰素-γ诱导脐血嗜碱粒细胞表达人类白细胞抗原Ⅱ类分子(英文)

被引：2

作者：

谢梅林

吴银根

机构：

[1] 苏州大学药学系药理学教研室,上海中医药大学附属龙华医院苏州,上海,中国

来源：

Acta Pharmacologica Sinica | 2002年 / 07期

关键词：

嗜碱粒细胞; HLA抗原; 粒细胞-巨噬细胞集落刺激因子; 干扰素-γ;

D O I：

暂无

中图分类号：

R96 [药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的:探讨嗜碱粒细胞是否可表达人类白细胞抗原(HLA)Ⅱ类分子.方法:采用密度梯度离心和免疫磁珠方法分离和纯化脐血嗜碱粒细胞,通过体外培养及粒细胞-巨噬细胞集落刺激因子(GM-CSF)和干扰素(IFN)-γ刺激20-60小时后,用流式细胞仪分析 HLA Ⅱ类分子表达的情况.结果:嗜碱粒细胞的纯度 ≥83.5％,在 GM-CSF10 μg/L或 IFN-γ100kU/L刺激后,其膜表面可表达HLA Ⅱ类分子,表达百分率在刺激后20小时较高,分别为10.2％±2.1％和11.3％±1.0％.结论:嗜碱粒细胞具有表达HLA Ⅱ类分子的潜在能力.

引用

页码：71 / 74

页数：4

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