慢性阻塞性肺疾病患者C-反应蛋白的变化及LPS诱导下人肺泡型细胞(A549)C-反应蛋白的表达

被引：7

作者：

吴尚洁

赵水平

机构：

[1] 中南大学湘雅二医院呼吸科,中南大学湘雅二医院心内科长沙,长沙

来源：

中南大学学报(医学版) | 2005年 / 02期

关键词：

慢性阻塞性肺疾病; A549细胞; 脂多糖; C-反应蛋白;

D O I：

暂无

中图分类号：

R563.9 [其他];

学科分类号：

摘要：

目的:观察人肺上皮细胞在细菌脂多糖(LPS)诱导下CRPmRNA的表达及CRP分泌。方法:收集临床慢性阻塞性肺疾病(COPD)患者同步晨痰和血浆,进行CRPELISA检测;对培养的人肺泡上皮细胞株(A549细胞)给予不同浓度、不同时间LPS刺激,同时设立对照组,细胞处理后提取RNA用于RT PCR。同时,对培养上清液进行CRPELISA检测。结果:COPD患者痰中CRP浓度明显高于同期外周血中CRP的浓度(P<0. 05 )。培养的A549在不同浓度和不同时间的LPS诱导下有CRPmRNA表达及CRP的分泌,且呈时间依赖性,与对照组比较有统计学意义(P<0. 05～0. 01)。结论:COPD患者呼吸道内可能存在CRP的自分泌;A549在LPS诱导下存在CRPmRNA的表达及分泌。

引用

页码：207 / 210

页数：4

共 7 条

[1]

A self-fulfilling prophecy:C-reactive protein attenuates nitric oxide production and inhibits angiogenesis. Subodh V,Chao-HW,Shu HL,et al. Circulation . 2002

[2]

Expression of C-reactive protein by alveolar macrophages. Dong Q,Wright JR. J Immunol . 1996

[3]

Inflammatory response and body composition in chronic obstructive pulmonary disease. Eid AA,Ionescu AA,Nixon LS. et al. American Journal of Respiratory and Critical Care Medicine . 2001

[4]

Expression of C-reactive protein in the human respiratory tract. Gould JM,Weiser JN. Infection and Immunity . 2001

[5]

Value of C-reactive protein measurements in exacerbations of chronic obstructive pulmonary disease. Dev D,Wallace DD,Sankaran R,et al. Respiratory Medicine . 1998

[6]

Why are patients with chronic obstructive pulmonary disease at increased risk of cardiovascular disease? The potential role of systemic inflammation in chronic obstructive pulmonary disease. Don DS,Paul SF. Circulation . 2003

[7]

Inflammatory markers of lower respiratory tract infection in elderly people. Albazzaz MK,Pal C,Berman P et al. Age and Ageing . 1994

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