去势大鼠血清骨钙素水平及意义

Objective\ \ To measure the level of osteocalcin (BGP) in ovariectomized rat and study its significance. Methods\ 20 female SD inbred rats were divided into sham ovariectomized (sham OVX) group and ovariectomized group (OVX). 18 weeks postsurgery, serum and 24 hour urine were collected to measure BGP by radioimmunoassay. Serum Ca, P concentration, alkaline phosphatase activity, urine Ca, P and hydroxyproline were also measured. Results \ Compared with sham OVX rat, the BGP level of OVX rats (0 98±0 14μg/L)was markdly lower than that of sham OVX rat (1 50±0 32μg/L, P<0 01),and serum Ca, P were also reduced. Serum AKP, urine Ca, P and HOP were significantly increased.Conclusion\ Reduced BGP of OVX rat suggests that there is reduced bone formation and declined bone turnover during a slow phase of bone loss in ovariectomized rat.\;