芍药苷对人胃癌SGC7901/VCR细胞增殖抑制作用及其机制研究

被引:22
作者
方申存
戴伟
吴昊
束永前
刘平
机构
[1] 南京医科大学第一附属医院肿瘤科
关键词
芍药苷; SGC7901/VCR; NF-κB; Bcl-2; Bax; 凋亡;
D O I
暂无
中图分类号
R735.2 [胃肿瘤]; R285 [中药药理学];
学科分类号
100214 ; 1008 ;
摘要
目的:探索芍药苷(paeoniflorin)对人胃癌SGC7901/VCR细胞增殖抑制及凋亡的影响,并研究其可能的分子机制。方法:应用不同浓度芍药苷作用SGC7901/VCR细胞48h后,采用四甲基偶氮唑蓝法(MTT)检测细胞增殖抑制率;流式细胞术(FCM)检测细胞凋亡率;免疫蛋白印迹技术(Western blot)分析Bcl-2、Bax及细胞核内NF-κBp65蛋白的表达;酶联免疫吸附试验(ELISA)进一步确定核内NF-κB的转录活性。结果:芍药苷对SGC7901/VCR细胞生长有明显的抑制作用并促进其凋亡,这种作用呈现剂量依赖性;Westernblot和ELISA均证实芍药苷对NF-κB有明确抑制作用;随着芍药苷浓度增加NF-κB和Bcl-2表达水平逐渐下调(P<0.01),但对Bax没有明显影响。结论:芍药苷可明显抑制SGC7901/VCR细胞的增殖,诱导其凋亡;其机制部分可能与阻断NF-κB通路所介导的Bcl-2分子上调有关。
引用
收藏
页码:636 / 640
页数:5
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