NF-κ B激活在兔急性心肌梗死再灌注后无复流中的意义

被引：3

作者：

吕媛 ^{[1
]}

陈韵岱 ^{[2
]}

骆景光 ^{[1
]}

杨秀秀 ^{[1
]}

田峰 ^{[1
]}

辛毅 ^{[1
]}

金泽宁 ^{[1
]}

吕树铮 ^{[1
]}

机构：

[1] 首都医科大学附属北京安贞医院心内科

[2] 中国人民解放军总医院心内科

来源：

现代生物医学进展 | 2010年 / 10卷 / 04期

关键词：

心肌梗死; 无复流; 炎症; NF-κB;

D O I：

10.13241/j.cnki.pmb.2010.04.001

中图分类号：

Q95-3 [动物学的研究与实验];

学科分类号：

摘要：

目的:观察心肌核因子-κB(NF-κB)在急性心肌梗死(AMI)再灌注后无复流的活化情况,探讨NF-κB促进无复流发生发展的作用机制。方法:24只新西兰大白兔随机分为假手术组(冠状动脉只穿线不结扎)和缺血再灌注组(结扎冠状动脉2小时,再灌注1小时),每组12只。采用凝胶阻滞迁移分析方法(EMSA)检测正常区、缺血区和无复流区心肌组织中NF-κB活性;ELISA法测定不同时点血浆中白细胞介素-6(IL-6)、超敏C反应蛋白(CRP)以及肿瘤坏死因子-α(TNF-α)的含量;光镜、电镜观察心肌组织病理变化。结果:(1)与正常区相比,缺血区和无复流区心肌组织中NF-κB活性异常升高(P<0.01)。(2)与结扎前相比结扎后2h、再灌注后1h血浆IL-6、CRP、TNF-α水平呈进行性升高(P均<0.05)。(3)NF-κB的活性与无复流面积、血浆IL-6、CRP以及TNF-α水平呈正相关(分别为r=0.844,P<0.01;r=0.682,P<0.05;r=0.687,P<0.05;r=0.893,P<0.01)。(4)无复流面积与血浆IL-6、CRP以及TNF-α水平呈正相关(分别为r=0.861,P<0.01;r=0.806,P<0.01;r=0.877,P<0.01)。(5)光镜及电镜结果显示无复流区的心肌组织损伤较缺血区更为严重。结论:急性心肌梗死再灌注后无复流现象的发生可能与局部心肌组织中NF-κB的过度活化有关,活化的NF-κB通过促进IL-6、TNF-α等炎症因子的表达,参与无复流的发生发展过程。

引用

页码：601 / 605

页数：5

共 21 条

[1] Searching for NF-kappaB-based treatments of ischemia reperfusion injury. Latanich CA,Toledo-Pereyra LH. Journal of Investigative Surgery . 2009
[2] The white blood cell count is an independent predictor of no-reflow and mortality following acute myocardial infarction in the coronary interventional era
Kojima, S
Sakamoto, T
Ishihara, M
Kimura, K
Miyazaki, S
Tei, CW
Hiraoka, H
Sonoda, M
Tsuchihashi, K
Yamagishi, M
Inoue, T
Asada, Y
Ikeda, Y
Shirai, M
Ogawa, H
[J]. ANNALS OF MEDICINE, 2004, 36 (02) : 153 - 160
[3] An oligonucleotide decoy for nuclear factor-κB inhibits tumor necrosis factor-α-induced human umbilical cord vein endothelial cell tissue factor expression in vitro
Wang, LL
Wei, WN
Hu, Y
Song, SJ
Yan, Z
[J]. BLOOD COAGULATION & FIBRINOLYSIS, 2004, 15 (06) : 483 - 490
[4] Tramadol reduces myocardial infarct size and ex-pression and activation of nuclear factor kappa B in acute myocardial infarction in rats. Zhang LZ,Guo Z. European Journal of Anaesthesiology . 2009
[5] Myocardial no-reflowin humans. Niccoli G,Burzotta F,Galiuto L,et al. Journal of the American College of Cardiology . 2009
[6] Effect of no-reflow during pri-mary percutaneous coronary intervention for acute myocardialinfarction on six-month mortality. Brosh D,Assali AR,Mager A,et al. The American Journal of Cardiology . 2007
[7] Enhanced inflammatory response via activation of NF-κB in acute experimental diabetic neuropathy subjected to ischemia–reperfusion injury[J] . Yanping Wang,Ann M. Schmeichel,Haruyasu Iida,James D. Schmelzer,Phillip A. Low. &nbspJournal of the Neurological Sciences . 2006 (1)
[8] 组织因子与无复流研究进展
吕媛
陈韵岱
[J]. 心肺血管病杂志, 2009, 28 (04) : 291 - 294
[9] The white blood cell count is an independent predictor of no-reflow and mortality following acute myocardial infarction in the coronary interventional era. Kojima S,Sakamoto T,Ishihara M. Annals of Medicine . 2004
[10] Baseline von Willebrand factor plasma levels and no-reflow phenomenon after primary percu-taneous coronary intervention for ST segment elevation myocardial infarction. Sgueglia GA,Niccoli G,Spaziani C,et al. International Journal of Cardiology . 2009

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