草鱼同工酶基因座位多态性的初步研究

被引:20
作者
吴力钊
王祖熊
机构
[1] 中国科学院水生生物研究所
关键词
电泳; 草鱼; 同工酶基因座位; 多态性;
D O I
暂无
中图分类号
学科分类号
摘要
采用垂直淀粉凝胶电泳及特异性组织化学染色技术研究了25尾草鱼的6种同工酶系统[ LDH ,MDH ,ADH, GDH, IDH, EST )约18—23个基因座位的遗传变异型。有7个基因座位(s-Mdh-A,Adh-B,Gdh-1,Gdh-2,Est-1,Est-6,Est-14)具有多态性,在其中4个基因座位(s-Mdh-A,Adh-B,Gdh-1,Est-1)上观察到的基因型频率与Hardy-Weinberg定律相符。实验表明草鱼的同工酶基因座位具有明显的多态性。这一结果为草鱼的人工选种和定向育种的可能性提供了依据。对草鱼GDH,EST同工酶的遗传基础、亚基组成以及酶变异的机理等问题进行了讨论。
引用
收藏
页码:116 / 124
页数:9
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