双农杆菌共转化获得无标记转pepc基因的水稻植株(英文)

被引：11

作者：

刘峰

赵伊英

苏永昌

许明

陈丽娜

郑金贵

不详

机构：

[1] 福建农林大学生命科学学院

[2] 福建农林大学生命科学学院福州

[3] 福州

来源：

应用与环境生物学报 | 2005年 / 04期

关键词：

pepc基因; 无选择标记; 双农杆菌/双质粒; 转基因水稻;

D O I：

暂无

中图分类号：

S511 [稻];

学科分类号：

0901 ;

摘要：

利用双农杆菌/双质粒的共转化系统获得了无选择标记转pepc基因的水稻植株.采用两个农杆菌EHA105转化粳稻品种台粳9号幼胚诱导的胚性愈伤组织,其中一个农杆菌内的质粒表达载体的T-DNA区仅含有目的基因pepc,另一个的T-DNA区含有选择标记基因hpt和GUS报告基因.获得抗性愈伤的转化率为9.2%.85.3%的抗性愈伤分化成T0代株系,其中78.8%的T0代植株为GUS阳性转基因植株.PCR分析表明,在78个T0代GUS阳性植株(来源于23个抗性愈伤组织)中共有35个植株(来源于7个抗性愈伤组织)含有pepc基因,即在23个GUS阳性株系中发生共转化株系的频率为30.4%.7个共转化的株系中有5个株系自交产生后代植株中含有无标记转pepc基因植株,其比例为71.4%.无标记的转pepc基因水稻植株中PEPC活性平均比对照提高8.8倍;与非转基因对照植株相比,转pepc基因水稻植株表现出更强的光合能力.图5表3参26

引用

页码：393 / 398

页数：6

共 13 条

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