大鼠脑缺血再灌注后神经元和星形胶质细胞凋亡的比较研究

被引：13

作者：

王萍

王伟

徐运兰

谢敏杰

杜一星

机构：

[1] 华中科技大学同济医学院附属同济医院神经内科

来源：

中国组织化学与细胞化学杂志 | 2006年 / 02期

基金：

国家自然科学基金重点项目;

关键词：

脑缺血; 凋亡; 神经元; 星形胶质细胞; 流式细胞术;

D O I：

暂无

中图分类号：

R741 [神经病学];

学科分类号：

1002 ;

摘要：

目的比较研究大鼠局灶性脑缺血再灌注后神经元和星形胶质细胞的凋亡规律。方法建立大鼠大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)再灌注模型,在缺血再灌注后1、3、7、14d断头取脑,应用流式细胞分选技术和原位末端标记法分别检测各组MCAO后不同时期神经元和星形胶质细胞凋亡情况。结果局灶性脑缺血再灌注后,海马区星形胶质细胞凋亡数量超过神经元,其凋亡以再灌注3d最为显著,而神经元则以7d最为显著;而皮层区神经元凋亡数量超过星形胶质细胞,两种细胞凋亡均在再灌注后7d达高峰。结论脑缺血再灌注后,皮层和海马区的神经元及星形胶质细胞均可发生凋亡,海马区星形胶质细胞比皮层区更易凋亡,而皮层区神经元比海马区更易凋亡。

引用

页码：113 / 118

页数：6

共 12 条

[1]

Correlation between cerebralblood flow and histologic changes in a new rat model ofmiddle cerebral artery occlusion. Nagasawa H,Kogure K. Stroke . 1989

[2]

Early detection ofDNA strand breaks in the brain after transient focal is-chemia:implications for the role of DNA damage in ap-optosis and neuronal cell death. Chen J,Jin K,Chen M,et al. Journal of Neurochemistry . 1997

[3]

Gap-junction-mediated propagation and amplification of cell injury. Lin JH,Weigel H,Cotrina ML,et al. National Neuroscience . 1998

[4]

Glial nitricoxide and ischemia. Gibson CL,Coughlan TC,Murphy SP. Glia . 2005

[5]

Temporal profile ofin situ DNA fragmentation after transient middle cere-bral artery occlusion in the rat. Li Y,Chopp M,Jiang N,et al. Journal of Cerebral Blood Flow and Metabolism . 1995

[6]

Differential sensitivi-ty of murine astrocytes and neurons from different brainregions to injury. Xu L,Sapolsky RM,Giffard RG. Experimental Neurology . 2001

[7]

Highsensitivity of protoplasmic cortical astroglia to focal is-chemia. Lukaszevicz AC,Sampaio N,Guegan C,et al. Journal of Cerebral Blood Flow and Metabolism . 2002

[8]

Astrocytes in cerebralischemic injury:morphological and general considera-tions. Panickar KS,Norenberg MD. Glia . 2005

[9]

Ischemia-induced pro-grammed cell death in astrocytes. Giffard RG,Swanson RA. Glia . 2005

[10]

Hy-poxia-ischemia causes abnormalities in glutamate trans-porters and death of astroglia and neurons in newbornstriatum. Martin LJ,Brambrink AM,Lehmann C,et al. Annals of Neurology . 1997

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