转基因番木瓜研究进展

被引：24

作者：

饶雪琴

李华平

机构：

[1] 华南农业大学资源环境学院

来源：

中国生物工程杂志 | 2004年 / 06期

关键词：

番木瓜环斑病毒; 外壳蛋白; 抗性;

D O I：

10.13523/j.cb.20040608

中图分类号：

S667.9 [其他];

学科分类号：

摘要：

番木瓜环斑病毒 (PRSV)使热带亚热带的重要水果番木瓜的生产受到严重影响 ,在众多方法防效不佳的情况下 ,利用病原获得抗性防治PRSV给番木瓜的生产带来了光明。综述了近年来转PRSVCP基因番木瓜中影响番木瓜转化因素和转基因番木瓜的抗性因素。转PRSV外壳蛋白 (CP)基因的番木瓜中多以胚性组织为转化材料 ,被转化材料的生理状态和基因型 ,是影响转化效率和转基因植株质量的主要因素。所获得的转基因番木瓜对PRSV的抗性在很大程度上依赖于接种PRSV与所转化PRSVCP基因的序列同源性、转基因拷贝数和所转基因的位置等。

引用

页码：38 / 42

页数：5

共 11 条

[1] Genetically engineered immunity to Papaya ringspot virus in Australian papaya cultivars
Lines, RE
Persley, D
Dale, JL
Drew, R
Bateson, MF
[J]. MOLECULAR BREEDING, 2002, 10 (03) : 119 - 129
[2] 华南番木瓜病毒病及环斑病毒株系的调查鉴定
蔡建和，范怀忠
[J]. 华南农业大学学报, 1994, (04) : 13 - 17
[3] STABLE TRANSFORMATION OF PAPAYA VIA MICROPROJECTILE BOMBARDMENT
FITCH, MMM
MANSHARDT, RM
GONSALVES, D
SLIGHTOM, JL
SANFORD, JC
[J]. PLANT CELL REPORTS, 1990, 9 (04) : 189 - 194
[4] Somatic embryogenesis and plant regeneration from immature zygotic embryos of papaya ( Carica papaya L.)[J] . Maureen M. M. Fitch,Richard M. Manshardt. &nbspPlant Cell Reports . 1990 (6)
[5] Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization of culture conditions and DNA-microprojectile delivery procedures[J] . A. Perl,H. Kless,A. Blumenthal,G. Galili,E. Galun. &nbspMGG Molecular & General Genetics . 1992 (2)
[6] TRANSGENIC PAPAYA PLANTS FROM AGROBACTERIUM-MEDIATED TRANSFORMATION OF SOMATIC EMBRYOS
FITCH, MMM
MANSHARDT, RM
GONSALVES, D
SLIGHTOM, JL
[J]. PLANT CELL REPORTS, 1993, 12 (05) : 245 - 249
[7] Stable transformation of barley tissue culture by particle bombardment[J] . A. Ritala,L. Mannonen,K. Aspegren,M. Salmenkallio-Marttila,U. Kurtén,R. Hannus,J. Mendez Lozano,T. H. Teeri,V. Kauppinen. &nbspPlant Cell Reports . 1993 (7)
[8] Herbicide resistant transgenic papaya plants produced by an efficient particle bombardment transformation method[J] . José L. Cabrera-Ponce,Ariadne Vegas-Garcia,Luis Herrera-Estrella. &nbspPlant Cell Reports . 1995 (1)
[9] Transgenic papaya plants from Agrobacterium-mediated transformation of petioles of in vitro propagated multishoots
Yang, JS
Yu, TA
Cheng, YH
Yeh, SD
[J]. PLANT CELL REPORTS, 1996, 15 (07) : 459 - 464
[10] Efficient transformation of papaya by coat protein gene of papaya ringspot virus mediated byAgrobacterium following liquid-phase wounding of embryogenic tissues with caborundum[J] . Ying-Huey Cheng,Jiu-Sherng Yang,Shyi-Dong Yeh. &nbspPlant Cell Reports . 1996 (3-4)

← 1 2 →