荧光原位杂交在喀斯特山地土壤硫酸盐还原菌检测中的应用

被引：10

作者：

张伟 ^{[1
]}

刘丛强 ^{[1
]}

刘涛泽 ^{[1
]}

陆婷 ^{[1
]}

张丽丽 ^{[2
]}

机构：

[1] 中国科学院地球化学研究所环境地球化学国家重点实验室

[2] 贵州师范大学理学院

来源：

微生物学通报 | 2008年 / 08期

关键词：

荧光原位杂交; 土壤; 硫酸盐还原菌;

D O I：

10.13344/j.microbiol.china.2008.08.021

中图分类号：

X172 [环境微生物学];

学科分类号：

摘要：

采用荧光原位杂交(Florescence in situ Hybridization,FISH)技术分析了喀斯特山地土壤硫酸盐还原菌(SRB)的数量和空间分布状况。运用16SrRNA特异性探针,结合DAPI全细胞染色和样品稀释,FISH技术准确、高效地原位检测出土壤剖面中SRB的数量和分布状况。结果显示,在所研究的土壤剖面各层均有SRB检出,最低为(0.8±0.4)×107个/g,最高为(6.2±1.3)×107个/g,平均为(2.7±1.2)×107个/g。FISH能同时对土壤中的SRB进行定性和定量分析,是研究环境中SRB时空分布的快速有效的检测技术。

引用

页码：1273 / 1277

页数：5

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