转基因抗草苷膦大豆对大鼠生理代谢的影响及外源基因水平转移研究

被引：9

作者：

赵志辉 ^{[1
]}

杨立桃 ^{[2
]}

艾晓杰 ^{[3
]}

张大兵 ^{[2
]}

邹思湘 ^{[1
]}

机构：

[1] 南京农业大学动物医学院

[2] 上海市农业科学院生物技术研究中心

[3] 上海交通大学农业与生物技术学院

来源：

南京农业大学学报 | 2006年 / 01期

关键词：

抗草苷膦转基因大豆; 大鼠; 代谢产物;

D O I：

暂无

中图分类号：

S565.1 [大豆]; S816.42 [豆类];

学科分类号：

摘要：

为了分析转抗草甘膦转基因大豆对大鼠生理代谢水平的影响及其内源和外源基因遗传转移的情况,选用32只SD大鼠,随机分成2组,分别饲喂含30%转基因抗草苷膦豆粕和含30%非转基因豆粕的大鼠配合饲料,连续饲喂30 d。试验结果表明,与对照组相比,试验组大鼠体重,血清中尿素氮(SUN)、葡萄糖、天冬氨酸转氨酶(GPT)和血清总胆固醇均无显著差异(P>0.05)。同时,利用定性PCR方法检测试验大鼠的血液、肌肉、肝脏、胰脏、肾脏、胃及肠道内容物中的转基因豆粕内源基因和外源基因。结果表明,在用含转基因豆粕的饲料饲喂的大鼠胃、肠道内容物中发现了转基因豆粕的内源基因和外源基因片段,而在其血液及各组织器官中则没有发现,说明短期内转基因豆粕的内源和外源基因在大鼠体内没有发生遗传转移。

引用

页码：77 / 80

页数：4

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