非离子型去垢剂TritonX-114提取两歧双歧杆菌的脂磷壁酸

被引:19
作者
乐军
胡宏
机构
[1] 上海市第一肺科医院检验科
[2] 重庆医科大学检验医学系
关键词
双歧杆菌,TritonX-114,脂磷壁酸;
D O I
10.13381/j.cnki.cjm.1997.04.002
中图分类号
Q939 [微生物分类学(系统微生物学)];
学科分类号
071005 ; 100705 ;
摘要
本研究利用非离子型去垢剂TritonX-114提取两歧双歧杆菌细胞壁的脂磷壁酸(Lipoteichoicacid,LTA),DEAE-Sephacel阴离子层析后,TritonX-114与LTA分开为两个峰,收集LTA洗脱峰,经紫外扫描、化学组分分析证实提取的LTA无明显蛋白质、核酸污染。被动血球凝集试验结果显示,LTA能自主粘附绵羊红血球,并能同抗LTA抗体或抗两歧双歧杆菌抗体结合而使血球凝集。我们采用的方法是分离细菌细胞壁LTA的有效方法,并可用于其他细胞壁两性大分子如脂聚糖及脂多糖的分离。
引用
收藏
页码:7 / 10
页数:4
相关论文
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