rAAV1载体介导外源性VEGF-165和Angiopoietin-1基因治疗大鼠脑缺血的疗效评价及其机制研究

被引:4
作者
赵英杰 [1 ]
李照建 [2 ]
王任直 [1 ]
魏俊吉 [1 ]
李桂林 [1 ]
赵浩 [1 ]
机构
[1] 中国医学科学院北京协和医院神经外科
[2] 青岛大学医学院附属医院神经外科
关键词
脑缺血; 血管内皮生长因子; 血管生成素; 基因治疗; 脑室途径; 腺相关病毒; 血管生成; 血脑屏障;
D O I
暂无
中图分类号
R743.3 [急性脑血管疾病(中风)];
学科分类号
1002 ;
摘要
为研究经脑室途径联合应用血管内皮生长因子(VEGF)和血管生成素(Angiopoietin-1,Ang-1),治疗大鼠急性脑梗塞的效果,并探讨治疗的机制,采用脑立体定向输注的方法,将rAAV1-VEGF治疗载体或者rAAV1-VEGF和rAAV1-Ang-1的混合治疗载体,通过侧脑室转染途径对大鼠大脑中动脉阻塞缺血再灌注模型进行基因治疗。观测VEGF和Ang-1蛋白表达、血脑屏障通透性、脑微血管密度等指标,并对大鼠进行神经功能行为评分等。结果显示,联合应用VEGF和Ang-1治疗急性脑梗塞可降低血脑屏障通透性,减轻脑水肿,增加缺血灶周围脑区的微血管密度,改善大鼠的神经功能。由此得出结论:联合应用VEGF和Ang-1基因治疗大鼠急性脑缺血,可保护脑细胞,促进新生血管生成,减轻脑水肿,改善大鼠的神经功能。
引用
收藏
页码:39 / 49
页数:11
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