银杏叶提取物对新生SD乳鼠心肌细胞缺氧损伤的影响

被引：2

作者：

闵宁斌 ^{[1
]}

唐治国 ^{[2
]}

黎璞 ^{[3
]}

王丽萍 ^{[1
]}

党亚茹 ^{[1
]}

机构：

[1] 陕西省渭南市合阳县人民医院内科

[2] 陕西省人民医院心内科

[3] 中国人民解放军第四军医大学唐都医院

来源：

现代生物医学进展 | 2012年 / 12卷 / 18期

关键词：

银杏叶提取物; SD乳鼠; 缺氧; 凋亡;

D O I：

10.13241/j.cnki.pmb.2012.18.009

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的:研究银杏叶提取物对缺氧状态下新生SD乳鼠心肌细胞的影响及其可能机制。方法:新生1天SD乳鼠心肌细胞原代培养并利用氮气培养箱模拟低氧构建乳鼠心肌缺氧体外模型。分为3组处理:对照组,缺氧组,缺氧+药物拮抗组。缺氧时间为12 h,通过免疫组化等检测方法,观察各组心肌细胞的损伤情况及心肌Bcl-2、Bax蛋白表达情况。结果:缺氧可以造成新生SD乳鼠心肌细胞凋亡的发生(hypoxia:75.21%±1.21%,control:1.38%±0.45%,P<0.05,n=20),并导致其表达凋亡抑制因子Bcl-2蛋白水平的显著降低(0.125 fold VS control group,P<0.05),促细胞凋亡因子Bax蛋白水平显著升高(3.011fold VS control group,P<0.05);而银杏叶提取物作用后可明显逆转新生SD乳鼠心肌细胞凋亡的发生(EGb761:23.17%±0.43%,hypoxia:73.13%±1.22%,P<0.05,n=20),并明显逆转Bcl-2(5.716 fold VS hypoxia group,P<0.05)、Bax(0.273fold VS hypoxia group,P<0.05)等蛋白的表达水平。结论:凋亡相关因子Bcl-2和Bax等参与缺氧致心肌损伤过程,导致心肌细胞凋亡,银杏叶提取物能降低心肌Bax表达,提高Bcl-2表达,从而保护心肌细胞,抑制凋亡。

引用

页码：3464 / 3468

页数：5

共 12 条

[1]

Double Knockdown of Prolyl Hydroxylase and Factor-Inhibiting Hypoxia-Inducible Factor With Nonviral Minicircle Gene Therapy Enhances Stem Cell Mobilization and Angiogenesis After Myocardial Infarction.[J].Mei Huang;Patricia Nguyen;Fangjun Jia;Shijun Hu;Yongquan Gong;Patricia E. de Almeida;Li Wang;Divya Nag;Mark A. Kay;Amato J. Giaccia;Robert C. Robbins;Joseph C. Wu.Circulation.2011, 11_s

[2]

Biphasic effect of EGb761 on simulated ischemia-induced rat BMSC survival in vitro and in vivo.[J].Shibao Li;Derong Tang;Zhiwei Xue;Zhifeng Zhang;Xiaojing Sun;Yuepeng Liu;Hongyan Dong;Xiaoxing Yin;Zhongming Zhang.Life Sciences.2011, 19

[3]

In vivo therapy of myocardial infarction with mesenchymal stem cells modified with prostaglandin I synthase gene improves cardiac performance in mice.[J].Wei-Shiung Lian;Winston Teng-Kuei Cheng;Chun-Chun Cheng;Felix Shih-Hsiang Hsiao;Jin-Jer Chen;Ching-Feng Cheng;Shinn-Chih Wu.Life Sciences.2011, 9

[4]

The novel role of mast cells in the microenvironment of acute myocardial infarction [J].

Kwon, Jin Sook ;

Kim, Yong Sook ;

Cho, Ae Shin ;

Cho, Hyang Hee ;

Kim, Jeong Sook ;

Hong, Moon Hwa ;

Jeong, Seo Yeon ;

Jeong, Myung Ho ;

Cho, Jeong Gwan ;

Park, Jong Chun ;

Kang, Jung Chaee ;

Ahn, Youngkeun .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2011, 50 (05) :814-825

[5]

Protective effects of Ginkgo biloba extract (EGb761) and its constituents quercetin and ginkgolide B against β-amyloid peptide-induced toxicity in SH-SY5Y cells [J].

Shi, Chun ;

Zhao, Lina ;

Zhu, Bilian ;

Li, Qianxi ;

Yew, David T. ;

Yao, Zhibin ;

Xu, Jie .

CHEMICO-BIOLOGICAL INTERACTIONS, 2009, 181 (01) :115-123

[6]

Hyperbaric oxygen preconditioning reduces ischemia–reperfusion injury by inhibition of apoptosis via mitochondrial pathway in rat brain.[J].J.-S. Li;W. Zhang;Z.-M. Kang;S.-J. Ding;W.-W. Liu;J.H. Zhang;Y.-T. Guan;X.-J. Sun.Neuroscience.2009, 4

[7]

Rutin from Lonicera japonica inhibits myocardial ischemia/reperfusion-induced apoptosis in vivo and protects H9c2 cells against hydrogen peroxide-mediated injury via ERK1/2 and PI3K/Akt signals in vitro [J].

Jeong, Jae Ju ;

Ha, Yu Mi ;

Jin, Yong Chun ;

Lee, Eun Ju ;

Kim, Ju Sun ;

Kim, Hye Jung ;

Seo, Han Geuk ;

Lee, Jae Heun ;

Kang, Sam Sik ;

Kim, Yeung Shik ;

Chang, Ki Churl .

FOOD AND CHEMICAL TOXICOLOGY, 2009, 47 (07) :1569-1576

[8]

Ginkgo biloba extract (EGb761 ? ) influences monoaminergic neurotransmission via inhibition of NE uptake; but not MAO activity after chronic treatment.[J].Christian J. Fehske;Kristina Leuner;Walter E. Müller.Pharmacological Research.2009, 1

[9]

The role of free radicals and nitric oxide in the ischemia–reperfusion injury mediated by acute bladder outlet obstruction.[J]..International Urology and Nephrology.2008, 1

[10]

Ethanol hyperpolarizes mitochondrial membrane potential and increases mitochondrial fraction in cultured mouse myocardial cells [J].

Mashimo, Keiko ;

Ohno, Youkichi .

ARCHIVES OF TOXICOLOGY, 2006, 80 (07) :421-428

← 1 2 →