橘、橙、柚皮中橙皮苷和柚皮苷含量的测定

To determine the content of hesperidin and naringin in the hull of citrus reticulata Blnco,citrus simenis（L.）Osbeck and citrus grandis（L.）Osbeck by the way of HPLC.With shimadzu vp-ODS（4.6 mm×150 mm,5 μm） colum and the mobile phase is CH3CN-（H3PO4.pH=3.5）(22︰78),the velocity of flow is 1.20mL.min-1and the detective wavelength is favourable 283 nm.The separate degree between the determination component and other components is favourable（R>1.5）,the linearity rang of hesperidin and naringin are 0.022μg1.80 μg and 0.023μg1.90 μg respectively,The average recoveries of hesperidin and naringin are 99.3%,97.6% respectively and their RSD are 1.4%,2.3% respectively.Conclusion:This method is convenient and easy,its result can be used to afford the feasibility to confirm material of the two active components.