抑制表达谷氨酸合酶基因对水稻碳氮代谢的影响

被引：6

作者：

卢永恩

罗风

杨猛

李香花

练兴明

机构：

[1] 华中农业大学生命科学技术学院作物遗传改良国家重点实验室国家植物基因研究中心(武汉)

来源：

中国科学:生命科学 | 2011年 / 41卷 / 06期

关键词：

谷氨酸合酶; 水稻; 共抑制; 碳氮代谢;

D O I：

暂无

中图分类号：

S511 [稻];

学科分类号：

0901 ;

摘要：

提高水稻的氮素利用率对农业生产极为重要,水稻谷氨酸合酶(GOGAT,EC1.4.1.14)被认为具有提高水稻氮素利用率的潜力,但该酶在水稻中的功能以及其对碳氮代谢的影响一直未被系统的报道.本研究以抑制表达谷氨酸合酶基因的转基因水稻为材料,结合谷氨酸合酶基因家族全生育期表达谱数据,研究该基因家族在水稻体内的功能及其对碳氮代谢的影响.结果表明,谷氨酸合酶家族基因成员的表达模式各不相同,具有明显的组织和器官特异性,表明其在体内行使着不同的功能.与野生型相比,抑制表达谷氨酸合酶基因的转基因植株的分蘖数、地上部干重以及单株产量显著下降.同时,转基因植株叶片中的硝酸盐、部分游离氨基酸、叶绿素、糖、糖磷酸以及吡啶核苷酸含量也显著降低,但游离铵、α-酮戊二酸以及异柠檬酸含量上升.分析表明,谷氨酸合酶在水稻的碳氮代谢过程中扮演着重要角色,是水稻氮素高同化过程中必不可少的因子.

引用

页码：481 / 493

页数：13

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