淫羊藿苷通过上调VEGF信号通路促进脑低灌注大鼠的血管发生（英文）

被引：13

作者：

李菲

张洋洋

蔡锐

刘波

石京山

龚其海

机构：

[1] 遵义医学院基础药理教育部重点实验室暨特色民族药教育部国际合作联合实验室

来源：

遵义医学院学报 | 2018年 / 41卷 / 05期

关键词：

淫羊藿苷; 血管新生; 一氧化氮; 血管内皮生长因子; 脑缺血; 大鼠;

D O I：

10.14169/j.cnki.zunyixuebao.2018.0109

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的观察淫羊藿苷对大鼠慢性大脑皮层低灌注后血管新生的作用。方法采用双侧颈总动脉结扎(BCCAO)制备大鼠脑低灌注模型,不结扎大鼠为假手术对照。术后9 d分组:假手术组、淫羊藿苷-60、BCCAO、BCCAO+淫羊藿苷-15、BCCAO+淫羊藿苷-30和BCCAO+淫羊藿苷-60组。各组大鼠灌胃相应剂量的淫羊藿苷或蒸馏水,连续28 d。采用免疫荧光染色,CD31抗体标记毛细血管密度;采用Griess方法检测大脑皮层一氧化氮(NO)的含量;采用Western blot方法检测血管内皮生长因子(VEGF)信号通路关键分子的蛋白表达水平及磷酸化程度。结果 BCCAO大鼠脑内CD31阳性标记的血管数量减少;大脑皮层NO浓度降低;VEGF信号通路下调。而给予淫羊藿苷治疗28 d后,能部分逆转上述改变,表现为增加缺血脑区毛细血管数量,提高大脑皮层NO浓度,上调VEGF信号通路。结论淫羊藿苷对慢性脑低灌注时血管新生具有积极的促进作用,该作用与增加受损大脑皮层NO浓度和上调VEGF信号通路有关。

引用

收藏

页码：533 / 538

页数：6

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