花粉管介导的转bar基因水稻植株的获得及其遗传

被引：14

作者：

赵凌

王才林

宗寿余

黄骏麒

龚蓁蓁

机构：

[1] 江苏省农业科学院粮食作物研究所

[2] 中国科学院上海生物化学研究所

来源：

中国生物工程杂志 | 2003年 / 08期

关键词：

水稻; 花粉管通道法; bar基因; 转基因植株; 遗传;

D O I：

10.13523/j.cb.20030820

中图分类号：

S511.03 [];

学科分类号：

摘要：

采用花粉管通道法将bar基因导入籼稻品系E32 ,得到对除草剂Basta具有抗性的转基因水稻。遗传分析表明外源bar基因在受体植株后代中呈单基因显性遗传 ,在世代间可以稳定遗传。目前已分离出抗性稳定的株系。

引用

页码：92 / 95

页数：4

共 18 条

[11] USE OF BAR AS A SELECTABLE MARKER GENE AND FOR THE PRODUCTION OF HERBICIDE-RESISTANT RICE PLANTS FROM PROTOPLASTS [J].

RATHORE, KS ;

CHOWDHURY, VK ;

HODGES, TK .

PLANT MOLECULAR BIOLOGY, 1993, 21 (05) :871-884

[12] REGENERATION OF HERBICIDE RESISTANT TRANSGENIC RICE PLANTS FOLLOWING MICROPROJECTILE-MEDIATED TRANSFORMATION OF SUSPENSION-CULTURE CELLS [J].

CAO, J ;

DUAN, XL ;

MCELROY, D ;

WU, R .

PLANT CELL REPORTS, 1992, 11 (11) :586-591

[13]

Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryonic callus. Vasil V,Castillo AM,Fromm ME,et al. Journal of Biotechnology . 1993

[14]

Generationoflargenumbersofindepen dentlytransformedfertilebarleyplants. WanY,LemauxPG. Plant and Physiology . 1994

[15]

PLANT MOLECULAR BIOLOGY[P]. 英国专利:GB9306726D0,1993-05-26

[16]

Transgenic sorghum plants via microprojectile bombardment. Casas A M,Kononowiez A K,Zehr U B,et al. Proceedings of the National Academy of Sciences of the United States of America . 1993

[17]

Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Cao J,Duan X L,Mcelory D,et al. Plant Cell Reports . 1992

[18]

Introduction of exogenous DNA into cotton embryos. Zhou GY,Wen J,Huang JQ,et al. Methods in Enzymology . 1983

← 1 2 →