A20基因抑制内皮细胞组织因子表达的研究

被引：6

作者：

朱楚洪

应大君

李黔宁

糜建红

张伟

孙建森

机构：

[1] 第三军医大学解剖教研室

来源：

中华老年心脑血管病杂志 | 2002年 / 06期

关键词：

基因; 内皮生长因子; 凝血致活酶; 脂多糖类; 血栓形成; 基因表达;

D O I：

暂无

中图分类号：

R346 [];

学科分类号：

摘要：

目的　探讨外源性A2 0基因在内皮细胞获得表达及对脂多糖诱导的内皮细胞组织因子表达的影响。方法　DOTAP脂质体介导的pCAGGSEHA2 0和pMAMneo基因转染 ,经G4 18筛选阳性单克隆 ,再用免疫荧光组织化学检测A2 0基因的表达 ,将转染A2 0基因的内皮细胞与未转染的内皮细胞分别加脂多糖 ,经原位杂交及免疫荧光组织化学检测组织因子基因的转录和表达 ,采用裂解内皮细胞一步复钙凝块时间测定法测定组织因子促凝活性。结果　成功构建了pCAGGSEHA2 0真核表达重组体 ,A2 0基因在经G4 18筛选后的内皮细胞中得到有效表达 ,能抑制 70 %组织因子的表达及凝块的形成。结论　A2 0基因能够明显抑制脂多糖诱导的内皮细胞组织因子的表达 ,在心血管血栓形成的预防和治疗中有重要作用

引用

页码：404 / 407

页数：4

共 8 条

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