陆地棉优质纤维QTL的分子标记筛选及优质来源分析

被引:13
作者
胡文静
张晓阳
张天真
郭旺珍
机构
[1] 南京农业大学作物遗传与种质创新国家重点实验室
基金
国家高技术研究发展计划(863计划);
关键词
陆地棉; 纤维品质; QTL; 来源;
D O I
暂无
中图分类号
S562 [棉];
学科分类号
摘要
利用陆地棉优质品系7235、渝棉1号做亲本,以7235×渝棉1号的F2与F2:3分离群体为材料,开展不同来源棉花高强纤维QTL微卫星标记筛选,为进一步进行优质纤维QTL聚合育种提供基础。通过5514对SSR引物对亲本进行多态性筛选,获得117个多态性标记,用其中80个标记构建了总长为1147.8cM的遗传图谱;应用复合区间作图法分析了该组合的F2单株和F2:3家系纤维品质性状,共检测到36个纤维品质数量性状基因座(QTL),其中与纤维长度、比强度、细度、伸长率及整齐度相关的QTL各6、8、7、8和7个,分别解释各性状表型变异的3.4%~14.4%、5.0%~42.4%、6.5%~11.7%、5.1%~19.4%和6.9%~14.0%。通过分析来源于7235和渝棉1号高强QTL的染色体分布,表明两亲本在D7、D8、D9染色体上都存在控制纤维比强度的QTL,其中存在于D7和D8染色体上来自双亲的QTL紧密连锁,成簇分布在染色体上的一定区间内。而在D7和D9染色体上也发现双亲完全相同的优质QTL,其优质供体可能来自陆地棉优质品系PD4381。进一步分析了获得的QTL在聚合育种中的应用潜力。
引用
收藏
页码:578 / 586
页数:9
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