氧化型低密度脂蛋白和普伐他汀对人脐静脉内皮细胞细胞间粘附分子-1表达的影响

被引：26

作者：

张新超

徐成斌

张彤

不详

机构：

[1] 北京大学人民医院心内科!北京

[2] 北京大学人民医院心内科!北京

来源：

中国动脉硬化杂志 | 2000年 / 03期

关键词：

脂蛋白,低密度; 内皮; 细胞间粘附分子-1; 动脉粥样硬化;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

摘要：

观察氧化型低密度脂蛋白对人脐静脉内皮细胞细胞间粘附分子 - 1表达的诱导及普伐他汀对它的抑制影响 ,以期探讨氧化型低密度脂蛋白致动脉粥样硬化的机制及普伐他汀可能的非调脂抗动脉粥样硬化作用。体外培养人脐静脉内皮细胞 ,分别加氧化型低密度脂蛋白 5 0mg/L、10 0mg/L、2 0 0mg/L及氧化型低密度脂蛋白 (10 0mg/L) +普伐他汀 (10 -4～ -6mol/L) ,孵育 12h、2 4h和 36h ,采用细胞酶联免疫吸附试验、流式细胞技术和反转录聚合酶链反应分别测定细胞间粘附分子 - 1蛋白水平及mRNA水平。结果发现 ,氧化型低密度脂蛋白呈浓度依赖性和时间依赖性诱导细胞间粘附分子 - 1蛋白表达及mRNA表达 ,而普伐他汀对其诱导作用有浓度依赖性和时间依赖性的抑制影响。研究表明 ,氧化型低密度脂蛋白诱导血管内皮细胞细胞间粘附分子 - 1的表达 ,可能代表了其在动脉粥样硬化形成与发展的一个关键环节致动脉粥样硬化的又一机制 ;普伐他汀抑制氧化型低密度脂蛋白诱导的血管内皮细胞细胞间粘附分子 - 1表达 ,可能是其独立于调脂之外的另一抗动脉粥样硬化作用。

引用

页码：229 / 232

页数：4

共 17 条

[1] Antiatherothrombotic properties of statins: implications for cardiovascular event reduction. Rosenson RS,Tangney CC. The Journal of The American Medical Association . 1998
[2] Native low density lipoprotein increases endothelial cell adhesiveness by inducing ICAM-1. Smalley DM,Li JH,Italiano ML,et al. Arteriosclerosis and Thrombosis . 1996
[3] An animal model to study local oxidation of LDL and its biological effects in the arterial wall. Calara F,Dimayuga P,Niemann A,et al. Arteriosclerosis and Thrombosis . 1998
[4] Co- expression of ICAM-1, VCAM-1, ELAM-1 and Hsp60 in human arterial and venous endothelial cells in response to cytokines and oxidized lowdensity lipoprotein. Amberger A,Maczek C,Jurgens G,et al. Cell Stress and Chaperones . 1997
[5] Lysophosphatidylcholine,a component of atherogenic lipoproteins, induces mononuclear leukocyte adhesion molecules in cultured human and rabbit arterial endothelial cells. Kume N,Cybulsky MI,Gimbrone MA Jr. The Journal of Clinical Investigation . 1992
[6] HMG-CoA reductase inhibitors decrease CD11b expression and CD11b- dependent adhesion of monocytes to endothelium and reduce increased adhesiveness of monocytes isolated from patients with hypercholesterolemia. Weber C,Erl W,Weber KSC,et al. Journal of the American College of Cardiology . 1997
[7] Inhibition of a4 integrin and ICAM - 1 markedly attenuate macrophage homing to atherosclerotic plaques in apoE-deficient mice. Patel SS,Thiagarajan R,Willerson JT,et al. Circulation . 1998
[8] Enhancement of monocyte adhesion to endothelial cells by oxidatively modified low- density lipoprotein is mediadted by activation of CDllb. Weber C,Erl W,Weber PC. Biochemistry and Biophysics . 1995
[9] Regulation of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 in human vascular smooth muscle cells. Couffinhal T,Duplaa C,Moreau C,et al. Circulation Research . 1994
[10] Modified low density lipoprotein and its constituents augment cytokine - activated vascular cell adhesion molecule-1 gene expression in human vascular endothelial cells. Khan BV,Parthasarathy SS,Alexander RW,et al. The Journal of Clinical Investigation . 1995

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