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CRISPR/Cas9介导的基因组定点编辑技术

被引：76
作者：
方锐
畅飞
孙照霖
李宁
孟庆勇
机构：
[1] 中国农业大学
来源：
生物化学与生物物理进展 | 2013年 / 08期
关键词：
人工核酸内切酶; 基因编辑; CRISPR; 基因打靶; CRISPR/Cas;
D O I：
暂无
中图分类号：
Q78 [基因工程（遗传工程）];
学科分类号：
071007 ; 0836 ; 090102 ;
摘要：
Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas)9系统成功被改造为第三代人工核酸内切酶,与锌指核酸内切酶(zinc finger endonuclease,ZFN)和类转录激活因子效应物核酸酶(transcription activator-likeeffector nuclease,TALEN)一样可用于各种复杂基因组的编辑.目前该技术成功应用于人类细胞、斑马鱼和小鼠以及细菌的基因组精确修饰,修饰类型包括基因定点InDel突变、基因定点敲入、两位点同时突变和小片段的缺失.由于其突变效率高、制作简单及成本低的特点,被认为是一种具有广阔应用前景的基因组定点改造分子工具.本文从CRISPR/Cas的研究历史、分类、作用机理以及基因定点修饰应用等方面进行简单介绍,希望能够为在这一领域的科研工作者提供参考.
引用
收藏
页码：691 / 702
页数：12
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